Genome Exploration Toolbox

Owing to the growing number of genome sequencing projects, large amounts of genomic data are now becoming available. This wealth of data is spurring research efforts that aim at the analysis and characterization of the identified genes (genomics).

Even more important, there is a major drive now under way to characterize the associated gene products (proteomics). This is a challenge, because it requires the expression of sufficient protein quantities from large numbers of genes to permit the desired analysis of protein function (PF) and practical protein performance (PPP) for each of these gene products. This is where IMEnz Bioengineering can help you, because IMEnz Bioengineering has developed approaches and technologies to solve these issues.

IMEnz Bioengineering has great expertise in the expression and characterization of genes. For that purpose a unique Genome Exploration Toolbox (GET) has been developed. This proprietary Genome Exploration Toolbox facilitates fast and efficient expression of genes in a selection of hosts including Gram-positive and Gram-negative bacteria and eukaryotic hosts.

The Genome Exploration Toolbox contains unique and efficient gene cloning systems for a range of micro-organisms. These enable the production of significant levels of proteins in selected hosts, which are then available for further characterization. IMEnz Bioengineering is fully equipped for the expression and production of proteins and enzymes at sufficient levels to enable application tests.

In practice, after obtaining target gene sequences, IMEnz Bioengineering selects the best host(s) for the cloning and expression of the target gene(s). Among the different characteristics taken into account are codon usage, cellular localization of the gene product (e.g. intra versus extracellular), post-translational modifications (e.g. glycosylation), and potential lethality of the protein to the host(s).
In addition, both constitutive and inducible expression systems will be used. If desired, the genes can be equipped with specific tags.

Subsequently, the genes will be cloned in the appropriate vector systems and transferred into the selected production organism(s). Expression levels will be assessed and, if needed, further improved.

The IMEnz Bioengineering Genome Exploration Toolbox contains the following components:

Organism

Extracellular
Gene Products

Regulation of gene expression

Constitutive

Inducible

Gram-positive bacteria

Lactobacillus plantarum

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Lactococcus lactis

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Bacillus subtilis

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Gram-negative bacteria

Escherichia coli

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Pseudomonas putida

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Eukaryotic host

Hansenula polymorpha *

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Hansenula polymorpha is capable of glycosylation and subcellular sorting.
In addition, this host has proven extremely useful for the high level production of eukaryotic membrane proteins.